Gene Therapy 101
Adult Stem Cell
Vector

Microarray and Genomics Cores

The Microarray Core provides researchers with the expertise and instrumentation necessary for RNA transcript profiling using Affymetrix’s GeneChip technology. Consultation on RNA extraction, experimental design, data analysis, and data mining options are available.  Since December 2000, the Core has helped researchers from around the world process over 3000 samples, resulting in numerous peer-reviewed scientific publications.

Available equipment includes the Nanodrop Spectrophotometer, Agilent Bioanalyzer 2100, Affymetrix GeneArray scanner, two GeneChip fluidics stations, a GeneChip Hybridization Oven 640, and a GeneChip bioinformatics system. The system includes a complete laboratory information management system (LIMS), which gives users direct access to their raw data, as well as the ability to use Affymetrix GeneChip Operating Software (GCOS) and GeneChip RNA Expression Analysis Software (GREX) or other third party data analysis packages on their own computers to analyze and query gene expression data.  In the near future, we will offer users the ability to use the new Exon and Tiling GeneChip arrays.

The Bioinformatics Center allows researchers to use the Rosetta Inpharmatics Luminator software to analyze their own data. Other available data analysis packages include GCOS and Data Mining Tools (DMT), GeneSpring, and BioConductor/R. The Bioinformatics Center also is developing a web service that users can use to mine their gene- and protein-related data, and the ability to add additional public as well as user’s databases.

Services:
DNA Array Analysis
Genome/Genechip Bioinformatics
GeneChip Hybridization
Laser Capture Microdissection (LCM)
Real-Time PCR
RNA Gene Expression Profiling

Location:
Louisiana State University Health Sciences Center - New Orleans
Louisiana State University Health Sciences Center - Shreveport  

© 2007 Louisiana Gene Therapy Research Consortium - last update 08/20/2008