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Microarray and Genomics Cores
The Microarray Core provides researchers with
the expertise and instrumentation necessary for RNA transcript
profiling using Affymetrix’s GeneChip technology. Consultation on RNA
extraction, experimental design, data analysis, and data mining options
are available. Since December 2000, the Core has helped researchers
from around the world process over 3000 samples, resulting in numerous
peer-reviewed scientific publications.
Available equipment includes the Nanodrop Spectrophotometer,
Agilent Bioanalyzer 2100, Affymetrix GeneArray scanner, two GeneChip
fluidics stations, a GeneChip Hybridization Oven 640, and a GeneChip
bioinformatics system. The system includes a complete laboratory
information management system (LIMS), which gives users direct
access to their raw data, as well as the ability to use Affymetrix
GeneChip Operating Software (GCOS) and GeneChip RNA Expression
Analysis Software (GREX) or other third party data analysis packages
on their own computers to analyze and query gene expression data. In
the near future, we will offer users the ability to use the new
Exon and Tiling GeneChip arrays.
The Bioinformatics Center allows researchers
to use the Rosetta Inpharmatics Luminator software to analyze their
own data. Other available data analysis packages include GCOS and
Data Mining Tools (DMT), GeneSpring, and BioConductor/R. The Bioinformatics
Center also is developing a web service that users can use to mine
their gene- and protein-related data, and the ability to add additional
public as well as user’s
databases.
Services:
DNA Array Analysis
Genome/Genechip Bioinformatics
GeneChip Hybridization
Laser Capture Microdissection (LCM)
Real-Time PCR
RNA Gene Expression Profiling
Location:
Louisiana State University Health Sciences Center - New Orleans
Louisiana State University Health Sciences Center - Shreveport
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