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Vector Core The Vector Core is dedicated to the construction and production of large and small-scale quantities of research/preclinical-grade recombinant- adenovirus, lentivirus, and adeno-associated virus vectors. Services provided by the Core include construction of adenoviral shuttle vectors, co-transfection of shuttle vectors with adenovirus helper DNA into viral producer cell lines, plaque purification of viral stocks, growth of high titer recombinant vector stocks and assay of recombinant and helper virus titers in stocks. In addition to development and construction of new vectors, the prime responsibility of the Core is controlled production of viral gene vectors for preclinical studies in animals. The Vector Core, in Shreveport, includes dedicated space for growth of infected and non-infected cells. This laboratory has a large capacity -80 oC freezer for storage of virus seed stocks and production lots of recombinant virus intended for preclinical studies. It also has access to a P-3 laboratory, which can be used for experiments in which containment of biohazardous material is required. The Vector Core, in New Orleans, also works on process optimization of vector production and purification. Great progress has been accomplished with the development of a lentivirus purification method using high performance liquid chromatography (HPLC), which may help improve gene transfer. Services: Locations: |
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